东北虎γ-干扰素基因的克隆、生物信息学分析及其在毕赤酵母中的表达     

翟俊琼  吴亚江  代军威  陈安妮  周妞  王晨  杜雪晴  罗满林  陈武 《中国畜牧兽医》2021,(1)

本试验旨在通过克隆东北虎γ-干扰素(IFN-γ)基因,研究其分子特征并预测蛋白生物学功能,为后续研究干扰素抗病毒活性做前期准备。通过RT-PCR从ConA诱导过的东北虎血淋巴细胞中扩增东北虎IFN-γ基因并测序,应用生物信息学方法进行序列分析。结果表明:东北虎IFN-γ编码区由504个核苷酸组成,共编码167个氨基酸,蛋白相对分子质量为19.59 ku,等电点为9.03,所编码的蛋白为碱性亲水性蛋白,其中前23个氨基酸可能为信号肽,IFN-γ编码蛋白保守结构域为IFN-γ超家族,且存在跨膜结构,其中1-6位氨基酸为胞内区域,7-28位氨基酸为跨膜区域,29-167位氨基酸为胞外区域;IFN-γ编码蛋白二级结构主要以α-螺旋(58.08%)和无规则卷曲(33.53%)为主,存在5个潜在的B细胞抗原表位,3个潜在的N-糖基化位点;分子进化分析显示,东北虎IFN-γ与GenBank上发表的东北虎、非洲狮、金钱豹、美洲狮、猎豹、家猫、加拿大猞猁、野猪等的核苷酸相似性为80.4%~99.8%,氨基酸相似性为70.5%~100%,东北虎IFN-γ与非洲狮、金钱豹亲缘关系最近,美洲狮、猎豹、家猫、猞猁次之,野猪最远。通过合成改造后的东北虎IFN-γ基因,构建能表达IFN-γ蛋白的重组质粒pPIC9K-IFN-γ,将其导入高效表达系统-毕赤酵母中进行诱导表达,经SDS-PAGE分析,表达蛋白的分子量约17.8 ku,与预期大小相符,表明东北虎IFN-γ成功表达。  相似文献   

Histopathology of Hematopoietic Tissue in Channel Catfish,Ictalurus punctatus,with Anemia     

《Journal Of Applied Aquaculture》2013,25(1-2):101-108

Outbreaks of anemia (CCA) in channel catfish, Ictalurirs punctatus, in Mississippi occur predominantly during spring and fall. Between October, 1990 and May, 1991, twenty-seven cases of CCA were submitted to the Mississippi Cooperative Extension Service-College of Veterinary Medicine Fish Diagnostic Laboratories. Histologic lesions were identified in the head and trunk kidneys of all affected fish. The head kidney contained less than 25% immature hematopoietic tissue in 3 of 22 cases (14%). 25-50% immature hematopoietic tissue in 7 of 22 cases (32%), and greater than 50% immature hematopoietic tissue in 12 of 22 cases (54%). The trunk kidney contained less than 25% immature hematopoietic tissue in 5 of 27 cases (18%), 25-50% immature hematopoietic tissue in 15 of 27 cases (56%), and greater than 50% immature hematopoietic tissue in 7 of 27 cases (26%). The histopathologic findings noted in cases of CCA from Mississippi affect primarily the hematopietic tissues of the head and trunk kidneys.  相似文献   

日粮和管理对东北虎血液指标及免疫功能的影响     

王兴金  赵云华  陈黎龙  黄志宏  李婉萍  李少基  谢高基 《野生动物》2007,28(1):21-24

从营养和管理方式均不同的两组东北虎群中各选5只体型适中的成年雄性东北虎作为研究对象，其中笼养组以纯肉为日粮，散养组以混合饲料为日粮。麻醉后采集静脉血，测定血液中各项生理生化指标，外周血淋巴细胞增殖性反应，以及血清中白细胞介素-2（IL-2）、白细胞介素-6（IL-6）和肿瘤坏死因子（TNF）的含量。结果：散养组外周血淋巴细胞增殖性反应显著高于笼养组（P〈0．05）；血清尿素、肌酐、淀粉酶、谷丙转氦酶、谷草转氦酶等指标在两组间差异显著（P〈0．05）：全血红细胞计数、白细胞计数、血红蛋白含量、血清蛋白、甘油三酯、总胆固醇等指标组间差异不显著（P〉0．05）。结果显示：营养和管理方式对东北虎某些血液指标产生影响，混合日粮和散养方式更有利于维持东北虎的正常免疫机能和健康。  相似文献   

东北虎朊病毒基因的克隆与序列分析     

吴长德  赵德明  杨建民  周向梅 《中国预防兽医学报》2007,29(3):190-193

根据已报道的哺乳动物朊病毒基因序列设计引物，采用PCR方法扩增了25只东北虎的朊病毒基因，克隆、测序及序列分析表明，所得到的东北虎朊病毒基因片段为402bp，编码134个氨基酸的前体蛋白，核苷酸序列同源性为99．67％。共发现了4个核苷酸多态性（T423C，A501G，C511A，A610G），其中C511A和A610G的碱基突变导致K171Q和A204T氨基酸的变异。与已报道的猫、貂、绵羊、鼠和犬等哺乳动物的氨基酸序列比较，结果与猫（AF003087，97．3％）和绵羊（97．3％）的氨基酸同源性最高。  相似文献   

Decreasing shrimp (Pandalus borealis) sizes off Newfoundland and Labrador – environment or fishing?     

P. A. KOELLER  C. FUENTES-YACO  T. PLATT 《Fisheries Oceanography》2007,16(2):105-115

During the 1990s, carapace length statistics including minimum size caught (L_min), mean male and female lengths, size at sex transition (L₅₀) and maximum size (L_max) of northern shrimp (Pandalus borealis) decreased in commercial and survey catches off Newfoundland and Labrador. Decreased growth rates caused by decreases in per‐capita food availability due to large population increases, exacerbated by increased metabolic demands from higher water temperatures in the mid‐1990s, appear to be the main cause of the size decrease. Fishing could have had an accelerating effect on environmentally driven decreases in shrimp growth and size by ‘cropping’ the largest shrimp from the population. The greatest decreases in shrimp size occurred in Hudson Strait and the adjacent northern shelf, the area which also has the highest densities and largest shrimp. We hypothesize that the greater size decrease here resulted from decreased primary production from decreased nutrient flux into the euphotic zone, caused by increased atmospheric warming, freshwater runoff and stratification during the warming trend of the 1990s.  相似文献   

Spatio-temporal distribution of sergestid shrimp Acetes intermedius in the coastal waters of southwestern Taiwan     

Wann-Duen Chiou  Chin-Cheng Wu and  Lei-Zong Cheng 《Fisheries Science》2000,66(6):1014-1025

SUMMARY: Spatio-temporal variation of the sergestid shrimp Acetes intermedius in the coastal waters of southwestern Taiwan was investigated based on 1546 samples collected by 13 commercial fishing vessels from July to October 1996. Adult sergestid shrimp started a down-estuary migration with the increased river discharge associated with heavy summer rainfall. Fishing season for this species was to commence after that. In July, the shrimp stock was found on the shallow continental shelf off Tungkang and Fangshan. They mainly aggregated in Kaoping Canyon off Tungkang during August and September occurring at very high densities. In October, the shrimp returned to the coastal waters off Tungkang and Fangshan at lower densities. The heavy rainfall is a factor that triggers the shrimps to make a down-estuary migration that could reduce competition for food between adults and their offspring during the period of high river discharge. When the northeast monsoon prevailed, the sergestid shrimp moved back to the estuaries and the fishing season was terminated.  相似文献   

用丰年虫卵壳制备甲壳素和壳聚糖     

孙文敏  李铮  李久红 《渔业现代化》2008,35(2):57-59

介绍了用丰年虫卵壳制备甲壳素及壳聚糖的方法及过程,通过傅立叶变换红外光谱仪的检测并与美国标准谱图库检索出的用蟹壳制备的壳聚糖光谱图对照,证实丰年虫卵壳制备的壳聚糖。  相似文献   

亚成体东北虎感染巴氏杆菌死亡一例     

邓爱怀  崔芳燕 《野生动物》2011,32(3):154-155

2009年1月11日,北方森林动物园发生一起自繁自养的亚成体东北虎感染巴氏杆菌死亡病例。本病例从发病到死亡共5 d时间,病程较短,以食欲下降至废绝,饮水量不变,精神沉郁,口腔腺体分泌增多、流涎,呈滴水样,腹部疼痛感明显为特征,口服诺氟沙星和多酶片治疗无效后改用肌注头孢哌酮钠和庆大霉素治疗,3 d后出现神经症状抽搐死亡。剖检肝脏表面有大量的针尖大小黄白色点状坏死灶。根据临床症状、病理剖检和实验室检查,确诊为巴氏杆菌感染。  相似文献   

壶瓶山自然保护区华南虎野外调查及栖息地评价   总被引：3，自引：0，他引：3  

黄祥云  胡德夫  唐小平  王志臣  刘伟石 《浙江林学院学报》2004,21(2):180-184

湖南省壶瓶山是森林和野生动物类型的国家级自然保护区,是近几年发现华南虎Panthera tigris amoyensis证据较多的区域.中美华南虎联合调查队于2001年10～12月采用访问调查法、样带调查法、痕迹调查法和红外线自动照相技术等目前国际国内通用的方法和技术,对该保护区进行了调查.此次调查虽未得到华南虎存在的确切证据,但较系统地调查了该区域的生境状况,初步评价了华南虎的生存环境.该区域历史上是华南虎分布较多的区域.经分析,目前仍具备华南虎生存的基本条件,是以后实施华南虎重引入工程较为理想的试验基地.表2参13  相似文献   

Contribution of microorganisms to the biofilm nutritional quality: protein and lipid contents     

C. FERNANDES da SILVA  E. BALLESTER  J. MONSERRAT  L. GERACITANO  W. WASIELESKY JR  P.C. ABREU 《Aquaculture Nutrition》2008,14(6):507-514

The nutritional quality of biofilm, a microbial community associated to an organic matrix, was evaluated in artificial substrate (polyethylene screen) in net cages during 30 days in the Patos Lagoon estuary, Southern Brazil. During this period, samples of biofilm were collected each 5 days for analysis of chlorophyll a, microorganisms abundance, dry weight, protein and lipid contents. During the study, chlorophyll a varied from 0.38 to 2.75 μg cm^?2; dry weight between 7.16 and 17.63 mg cm^?2; protein content from 0.43 to 1.76 mg cm^?2 and lipid concentration between 1.21 and 4.23 mg cm^?2. The variation of lipid in the biofilm was closely related to the abundance of free heterotrophic bacteria (34.25–56.54 × 10⁶ cells cm^?2), filamentous cyanobacteria (7.5–15.9 × 10⁶ filaments cm^?2), flagellates (6.92–12.89 × 10⁶ cells cm^?2) and mainly nematodes (29–1,414 organisms cm^?2), while protein content varied similarly to the abundance of unicellular centric diatoms (52.10–179.81 × 10³ cells cm^?2), and nematodes. This information will allow a better management of food supply to raised aquatic organism with the utilization of natural productivity in the culture systems, with considerable decrease in production costs.  相似文献